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Forensic Medicine · Sexual offences and forensic psychiatry: examination of the accused
Sample itemA man is arrested six hours after an alleged rape and the doctor wishes to show on his body evidence of recent vaginal contact. Which specimen and test is appropriate?
Original practice item, not a recalled exam question. Nothing here is recorded.
The answer and the reasoning
D. Glans swab examined for glycogen-rich vaginal epithelial cells with iodine
Vaginal epithelial cells are rich in glycogen, so cells recovered from the glans and coronal sulcus stain mahogany brown with iodine. This points to recent vaginal contact. His own semen would confound sperm or spermine tests, and a gonococcal culture is unrelated.
Option by option
A. Penile skin tested with Barberio's reagent
Barberio's test detects spermine in semen, which could be his own and is not specific for vaginal contact.
B. Glans swab examined for spermatozoa after Gram staining
Sperm on his glans may be his own and would not show contact with the survivor.
C. Urethral swab cultured for gonococci
A gonococcal culture detects infection, not recent intercourse.
D. Glans swab examined for glycogen-rich vaginal epithelial cells with iodine (correct)
Correct. Glycogenated vaginal cells on the glans stain brown with iodine and indicate recent vaginal contact.
Source note: Original NextPrep sample item — pending expert review. This item is original practice material awaiting independent expert review, so confirm important facts in a standard textbook.
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